Equipment & Forms
Booking Instructions & Reservation Policies
Please read our policies regarding reservations, cancellations, late starts, and overruns before booking.
- Reservations are managed on a “First Come, First Served” basis.
Cancellation Policy
- Cancellations must be made at least 24 hours in advance.
Late Start
- Please respect the scheduled time of other users and make every effort to adhere to your reservation.
- If you are delayed by more than 30 minutes, you must email Dr. Jiakai Hou (jhou21@central.uh.edu) to confirm and secure your reservation.
- If there is a delay of more than 30 minutes without prior notice, your reservation will be automatically canceled, and a 1-hour charge will be applied.
Overrun
- Extension beyond your reserved time is only permitted if the system is available and does not conflict with the next scheduled user.
- Please check the schedule or consult facility staff before overrunning your session.
Available Instruments
BD LSRFortessa™ X-20 Cell Analyzer with High-throughput Sampler
- 5 spatially separated lasers (355/405/488/561/640 nm)
- High Multiplexing Capability (up to 18 parameters)
- Automated Fluidics System-BD High Throughput Sampler
- Efficient Optical Design
- BD LSRFortessa™ X-20 Standard Operation Procedure
- BD LSRFortessa™ X-20 User Agreement
- BD LSRFortessa™ X-20 User Guide
- 5-laser, 18-color
- Laser: 355 (Ultra Violet)
- Filter 379/28 BP: Hoechst Blue, BUV396
- Filter 520/50 BP: BUV496, BUV563
- Laser: 405 (Violet)
- Filter 450/50 BP: Alexa Fluor 405, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Violet, BV421, DAPI
- Filter 525/50 BP: Alexa Fluor 430, AmCyan, CFP, Live/Dead fixable Aqua, BV510
- Filter 610/20 BP: BV605, Pacific Orange, Qdot 605
- Filter 670/30 BP: BV650, Qdot 655
- Filter 710/50 BP: BV711, Qdot 700
- Filter 780/60 BP: BV786, Qdot 800
- Laser: 488/10 (Blue)
- Filter 530/30 BP:Alexa Fluor 488, FITC, GFP, Sytox Green, Calcien Green
- Filter 710/50 BP: PerCP, PerCP-Cy5, PerCP-Cy5.5, 7-AAD
- Laser: 561(Yellow Green)
- Filter 586/15 BP: PE, DS Red, PI
- Filter 610/20 BP: PE-Texas Red, PE-Alexa 610, PE-CF594, Live/Dead Fixable Red, Alexa Fluor 610, mCherry, mKate, tdTomato, PE/Dazzle 594
- Filter 670/30 BP: PE-Cy5
- Filter 710/50 BP: PE-Cy5.5, PE-Alexa 700
- Filter 780/60 BP: PE-Cy7
- Laser: 640 (Red)
- Filter 670/30 BP: APC, Alexa Fluor 647, Alexa Fluor 680, Sytox Red
- Filter 730/45 BP: Alexa Fluor 700, APC-Alexa 700, APC-R700
- Filter 780/60 BP: APC-Cy7, APC-H7
- Software Version:
- BD FACSDiva 8.0
- Sample Acquisition:
- 12mm x 75mm polystyrene tubes, HTS plate reader (96 well plates)
BD FACS Melody™ Cell Sorter
- Automated Cell Sorting
- High Purity & Recovery
- Aerosol Management System (AMS)
- Multi-Sample Compatibility
- BD FACS Melody™ User Guide
- 1-laser, 4-color
- Laser: 488/10 (Blue)
- Filter 527/32 (507LP): Alexa Fluor 488, FITC, GFP, BD Horizon Brilliant blue 515, Sytox Green, Calcien Green
- Filter 585/42 (560LP): PE, PI
- Filter 700/54 (665LP): PerCP, PerCP-Cy5.5, 7-AAD, BD Horizon Brilliant blue 700
- Filter 783/56 (752LP): PE-Cy7
- Software Version:
- BD FACSChorus
- Sample Acquisition:
- 12mm x 75mm polystyrene tubes
Applied Biosystems™ 7500 Fast Real-Time PCR System
Book Time On This Instrument (two instruments available): Booking Link 1 | Booking Link 2
- 5-Color Detection
- Fast Thermal Cycling
- High Sensitivity and Accuracy
- Built-in Calibration & Normalization
- Compatible with Various Chemistries
- Applied Biosystems™ 7500 User Guide
RS 2000 X-ray Irradiator
- High-Energy X-ray Source (Rate: 0.01-95Gy/min ,Time: 1-9999s)
- Precision Dose Delivery
- Programmable Dose Control
- Lead-Shielded Chamber
- Non-Radioactive Alternative
- Multi-Sample Capacity
- RS 2000 X-ray Irradiator Standard Operation Procedure
- RS 2000 X-ray Irradiator User Guide
Excelsior™ ES Tissue Processor
- Automated & Walk-Away Processing
- Capacity: Up to 300 cassettes in a single run
- Rapid & Gentle Processing
- User-Friendly Touchscreen Interface
Leica CM 1550 Cryostat
- Precise Temperature Control: down to -40°C for consistent sectioning
- Fast Freezing with Peltier Element
- Anti-Roll Plate & Heated Blade Holder
- Large Storage Capacity
- UV Disinfection System
Thermo Scientific™ HM 355S Automatic Microtome
- Fully Motorized Sectioning
- Adjustable Cutting Speeds
- Precision Section Thickness Control: 0.5 to 100 µm sections
- Memory Function
- Specimen Retraction Feature
- Universal Blade Holder
Keyence BZ-X810 Inverted Microscope
- Fully Automated System
- High-Resolution Fluorescence Imaging
- Brightfield & Phase Contrast
- Live-Cell Imaging
- Deep Imaging with Z-Stacking
- Tiling & Stitching
- Automated Quantification